Tao Sun

5 Questions 7 Answers 0 Followers

Questions related from Tao Sun

Is " Mouse malonyl coenzyme A ELISA Kit"suitable for bacteria?

I want to measure the malonyl-CoA in bacteria (not using HPLC). As far as I know, only kit for mouse or human is avaliable. So I just want to know if the "Mouse malonyl-Coa kit" also suits for...

03 March 2017 5,328 7 View

Could ROCHE Hybridization Buffer (cat. 11717472001) be directly used as Prehybridization and Hybridization solution for Southern Blot?

Long time ago, I bought ROCHE Hybridization Buffer (cat. 11717472001), now I want to do the SB assay, but i can't find any instructions about this buffer. So I wonder if this buffer was...

08 August 2016 4,747 4 View

No results: 3' RACE for bacteria using primer F and oligo dT ?

I extracted the total RNA from cyanobacteria and adding poly A using poly A polymerase, then synthesized cDNA using Thermo's kit. When using specific forward primer and oligo dT primer to amplify...

08 August 2016 2,339 1 View

Could I use a single promoter to express three proteins amplified from start codon ATG to stop codon TAG and fused together using overlapping PCR?

Three genes amplified from start codon ATG to stop codon TAG. If I fused them into one single fragment using overlapping PCR and cloned them into a vector under a single promter, could I get three...

07 July 2016 7,945 3 View

Which is the first reference using cyanobacteria as a platform for the production of biofuels or chemicals?

a reference

09 September 2014 2,775 0 View