I have found highly increased NO emission in Arabidopsis thaliana roots, but not significative changes in H2O2 production. How could I explain this? Which one could be its physiological meaning? Thanks!
NO and H2O2 are two different and independent signalling molecules in plants. There is lot of cross talk between two of them, that does not mean they have to produce at same time and amount. Each of it has its own signaling and synthesis mechanism.
hello,
To complete Venkat comment, the interplay between NO and ROS is quite complicated as RNS and ROS are also linked at multiple levels: at the chemical level through the formation of peroxynitrite (and other species), in terms of production (for instance NO down-regulates NADPH oxidase by S-nitrosylation as shown by the group of Gary Loake) and probably by acting on same protein targets (as shown for GAPDH or the chaperon CDC48). Furthermore, NO and H2O2 are able to regulate common genes as independent genes.
Generally, NO and ROS are produced simultaneously. The absence of H2O2 is therefore quite surprising but, of course, it might depend on the stimuli applied and, technically, on the method used to check the productions (selectivity and sensitivty of the method).
I suggest you to try another method for measuring H2O2 before giving a final conclusion.
Please find enclosed papers in which the NO/ROS cross-talks have been investigated or discussed (the PCE paper is now online).
All the best, David
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