Need to differentiated PC12 cells into neurites with NGF. Many type of NGF which one best and why the cell dead?

Ahmed Rayshan @Ahmed-Rayshan

31 May 2024 1 594 Report

Need to differentiated PC12 cells into neurites with NGF. Many type of NGF which one best and why the cell dead

Samir Ranjan Panda

Hi Ahmed Rayshan

To differentiate PC12 cells, use NGF at a dose of 50 ng/mL for 5-7 days in reduced serum medium (2% FBS).

Please find the link to NGF that I use.

https://www.sigmaaldrich.com/IN/en/product/sigma/n2513

Thanks,

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