Need Suggestions
I am doing kinetics assays and binding studies of my protein which is an enzyme .There are two substrates for this enzyme: sub-A and sub B. I am getting discrepancies in the Kd and Km values. The sub-A has Kd: 54 and Km : 155 value. Sub-B: Kd: 23, Km: 25. The IC50 of SubA: 1.27 micro molar and Sub B: 27 micro molar. Please also provide the references.
Hi there,
Could you please provide the actual values you obtained for both Km and Kd?
Hi again,
So kinetics with substrate B follows the rapid equilibrium hypothesis as Km~Kd (which means kcat
Thanks Dominique, Here is the Kcat
Inhibitor D + Sub A- 5.3 x 10xpower6 (Enzyme conc-15nm, Vmax- 0.08 M/min)
Inhibitor D + SubB- 2.9 x 10xpower8 (Enzyme conc-15nm, Vmax-4.3 M/min)
MOE binding affinity(kcal/mol)
Sub A -12.63
Sub B-11.12
Hi again,
What is inhibitor D? You did not mention it earlier... Do you mean that Km and kcat have been determined in presence of D? What about in absence of D?
- Badges
- Science topic
- Similar topics
- Biological Science
- Biochemistry
- Enzymology
- Enzymes