20 September 2016 1 7K Report

I need to design primer for inverse PCR site directed mutagenesis where primers are not complementary to each other and anneal back to back at 5' end (not a single base is complementary in primer)

In attached Image I am interested for strategy (A) substitution of base by mutagenesis. So how to design primer for this strategy also provide detailed protocol for this kind of mutagenesis ?

What is the success rate for 1 bp substitution by using this mutagenesis strategy? 

I am not getting whole information for this so please help .

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