Is the NBT test useful as a critical methodology for phagocytosis in the transition period in dairy cows or not?
I've not seen or heard of it used for dairy cows. If you are trying to quantify phagocytosis in cattle, I highly suggest the pHrodo kits (flow cytometric). You can analyze phagocytes under a light microscope but it is extremely time consuming and the time nullifies the cost. I had great success with the pHrodo kits in beef animals, collaborators have used it in transition dairy animals as well. If you need to store your cells for a day or two before analysis (e.g. you are using a core lab cytometer have have to book ahead of time) I have used the pHrodo dyes with formaldehyde fixation and had great results. If this is something you want to try send me a message and I can forward a protocol.
Goo day, thanks for your reply. I will recommend this to my professor and if we had access to the kit I will surely send you message. thanks again
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