I propagated the IBDV virus in the SPF eggs via the CAM route. The amount of harvested virus is limited so I want to ask if is there anyone with experience in doing realtime PCR instead of doing actual titration in eggs.
Hi, what is the actual question here? How to set up an assay to quantify IBDV?
Hi, Thanks for your answer. yes. I want to use the IBDV for animal trial, and I want to know if I can use qPCR instead of doing EID50
I think you can do it, indeed. These are examples of qPCR for IBDV:
https://pubmed.ncbi.nlm.nih.gov/16678230/
https://pubmed.ncbi.nlm.nih.gov/30447279/
Article Detecting Infectious Bursal Disease Using a VP1 Gene-Based R...
Cheers
Luigi
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