I have been doing SOD assay using riboflavin, NBT, Methionine in K-phosphate buffer pH 7.6 and accidentally I forgot to add EDTA in the reaction mixture, as a result the blue colour generation in the control mix (all components EXCEPT the enzyme extract) under light did not develop blue colour but very slowly and to very low level. when I repeated it and added EDTA I found the blue colour of the control mix (without enzyme extract) to develop fast as usual. Can anybody tell the role of EDTA in the oxidation of NBT and/or generation of superoxide from riboflavin? NOTE: The question here is not the effect of EDTA on SOD activity since control mix has NBT, riboflavin, methionine in pot. phosphate buffer pH 7.6
Thanks Dibakar Pal ... the second article shows that EDTA takes part in superoxide anion production
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