Desialylation of erythrocyte glycocalix has been postulated to play a role to enhance erythrocyte snatching at the spleen in sepsis. What biochemical/biophysical techniques could be best used to demosntrate the occurence of alterations in erythrocyte glycocalix caused by desialylation? Any hints involving cross-link reagent approaches?
Relevant reference:
Piagnerelli M., Zouaoui Boudjeltia K., Vanhaeverbeek M. (2006) Red Blood Cell Desialylation in Critically III Patients: An Underestimated Cause of Anemia. In: Vincent JL. (eds) Yearbook of Intensive Care and Emergency Medicine. Yearbook of Intensive Care and Emergency Medicine, vol 2006. Springer, Berlin, Heidelberg
Have you considered phase partitioning? Counter current distribution is I believe quite sensitive. Authors to look for: Derek Fisher and Cristina Delgado used to work on PEG dextran phase systems (but largely for affinity phase partitioning).
Good luck.
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