NADPH oxidase activity assay with cytochrome C?

18 September 2013 1 3K Report

I want to quantify the activity of NADPH oxidase in tissue homogenate using cytochrome C and NADPH. I am searching for a full protocol.

Kenza HAMMOUDI

Thanks for the link

Badges
Science topic

More Kenza HAMMOUDI's questions See All

For what reasons, and in what ways, did the Civil Rights movement in the United States become more radical between 1965 and 1968?

25 April 2023 3,334 1 View

What are the best softwares for studying the chemical-physical properties interactions between montmorillonite and anionic polymer ?

What are the best programs / softwares for studying the chemical-physical properties interactions between montmorillonite and anionic polymer

26 March 2023 7,136 1 View

Hello,everybody! Is " research laboratory on didactics " grammatically correct or not? Shouldn't we replace ON by OF?

Thanks!

20 May 2022 4,255 6 View

How to prepare and store spheroid 3d culture lysate for elisa ?

Hello, I'm looking for the best protocol to store and prepare lysate of 3d spheroid of cancer cells. Thanks

15 November 2021 2,869 0 View

TEM preparation for Mg?

TEM FOR Mg I need some informations about Tem sample preparation for magnesium, did someone worked on this before?? Thank you!!

28 April 2016 177 3 View

What's the difference between the "Orientation" and the "Misorientation "of the grain?

12 April 2016 746 4 View

Is there a mathematical model for ligament-to-bone attachment regarding the gradation of Young modulus?

Some sources explain there are 4 areas composing the ligament-to-bone or tendon-to-bone insertion, namely bone- calcified fibrocartilage - uncalcified fibrocartilage- ligament. I would like to...

07 October 2015 5,685 1 View

Food retailers and production systems - any thoughts?

Do you think that modern food retailers can contribute to the organization of production systems in developing countries and a positive change in their production practices and productivity?

12 August 2014 1,884 3 View

How to prepare, using a simple technique, a PbO2 electrode for use in anodic oxidation?

The electrode should be chemically very stable (used in very acidic and corrosive medium) and presenting an overvoltage higher than that of oxygen production.

14 January 2014 8,272 1 View

Could anybody tell me how we can measure COD or total carbon of a solid? What are the best and the easiest methods?

I am looking for methods that don't need lixiviation

03 January 2014 6,270 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View