Hi,
We are suspecting mycoplasma infection in our lab. We have already extracted DNA according to ATCC's mycoplasma detection kit 90-1001k. This kit says to do 2 rounds of PCR and it supplies '1st and 2nd primers'.
This kit is very old and we are having difficult time finding the right protocol.
Why do we do the second round of PCR?
Also, do we run agarose gel after the first round and purify amplified product? And feed that for the second round?
Does anyone have experience with this kit? Please tell us how to use the 2nd primer kit. Thanks so much! :)
Why waste time with an old kit, resulting in results which cannot be trusted, rather than get a new one?
the attached protocol will help. other information sheets about this kit are found at
http://www.lgcstandards-atcc.org/~/media/DBCFEEC877EE45FCB6D8D66729CDDD28.ashx
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