My SDS gel takes 4-5 hours to run completely. Can someone please help me understand what might be going wrong?

10 June 2020 1 7K Report

I am performing DNA-Protein Binding assay using Dyna beads. In order to check the bound protein i have to run SDS PAGE. the problem I am facing is my gel takes 4-5 hours to run completely. I have tried it with fresh running buffers as well as fresh buffers for gel casting. Can someone please help me understand what am I doing wrong to my gel to take such a long time to complete its run?

Roger Davis

Hi Tejosmita,

In addition to preparing fresh buffers, please select the appropriate gel concentration according to the molecular weight of the target protein. It is recommended to use a voltage of 120-200 V, and if the voltage is too low, small molecular weight protein bands will diffuse. It usually takes me about half an hour to run a piece of gel.

Hope it helps!

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