Hello everyone, greeting, hope you are doing well, I need some significant information, which is related to the use of silver nanoparticles in the aquatic ecosystem. My question is this when we apply different concentrations of Ag-NPs on Ciliates to check the toxicity of silver nanoparticles. We know that silver nanoparticles kill the ciliates, disturb the aquatic ecosystem. Anxiously waiting for your kind response. Thank You
Md. Masud Parvez.
Hello Md. Masud Parvez ,
Am finding it difficult to comprehend exactly what you want to know.
Is it the time of application or how to apply?
Dear Md. Masud Parvez ,
Although, it depend on your objectives, otherwise your expectations from using different AgNP concentrations to asses the toxicity of such Nanoparticles to ciliates. However, you have to bring in mind that increasing the concentration in aquatic system would forcefully increase agglomeration rates either auto-agglomeration or hetero-agglomeration, that means yo will increase the potential formation of bio-eco-corona, which forcefully will lead to an increase in the dynamic size of your particles, thus decreasing the bio-availability, in case that you aim to use natural entry into organisms.
Otherwise, it might be possible that you want to know which is the most suitable moment to add your AgNPs to the exposure media, that means that you have other considerations to take into account dependently on the type of exposure (in vivo as exemple...etc).
Furthermore, its important to highlight, that in general when there is no previous studies have been done using the model-organism, nor the pollutant that you aim to use, you may use to determinate the LC50, which also presents some controversies among nanotoxicity community, because the matter of the bio-availability, which may be reduced once the concentration (i.e. mass concentration) have been increased.
I hope that this helps you, otherwise please do not hesitate to ask for any further information that you feel may helps you.
Best wishes,
Younes,
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