My MTT result show cell viability more than 100% ?

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Adam B Shapiro

First, is this difference in absorbance statistically significant and reproducible?

If so, then you may be able to conclude that the treatment enhanced cell growth or survival.

Benjamín Cartes Saavedra

MTT can be influenced by cell growth but also by mitochondrial metabolism. If the cells had a increased metabolism it is not rare found an increase in the reduction of MTT salt.

Bjørn Petrat-Melin

Adding to Benjamin's comment above: if your treatment is stressing the cell you are also likely to see an increase in metabolims as a response.

Sudip Kumar Dutta

Thanks all for your valuable comments