In syngeneic or haplotype-matched mouse experiments with mixed gender (male and female) recipients, how much does the sex of the donor mouse matter? In your experience, is sex-mismatch not as major a determinant as haplotype-mismatch?
- Male > Male and Female > Female will obviously be the best choice.
- But what about Male > Female and Female > Male; which one is preferable for long term engraftment and prevention of GVHD?
- e.g.- female bone marrow engrafted into male recipient would reconstitute a female immune system that attacks H-Y on male host cells?
Dear Samarth,
These are good questions.
Male > Male and Female > Female will obviously be the best choice.
As in human stem cell transplantation Male > Male, Female > Female and Male > Female shout be better as Female > Male transplantations.
But what about Male > Female and Female > Male; which one is preferable for long term engraftment and prevention of GVHD?
Male >Male, Female > Female or Male > Female shout be better as Female > Male transplantations for long term engraftment and prevention of GvHD.
e.g.- female bone marrow engrafted into male recipient would reconstitute a female immune system that attacks H-Y on male host cells?
Yes, I would expect this.
The female transplant could contain memory cells against antigens (like HY-antigen) on male cells, too.
Best regards,
Thomas
Hi Samarth,
I'd suggest contacting Scott Russo, Georgia Hodes, or someone in their labs about this. Scott and Georgia have experience with this.