I have been trying to coat MnCO3 microparticles having isoelectric point at pH 7.6 with different biopolymers in aqueous medium.
1. As per my understanding MnCO3 should have positive charge berlow pH 7.6, correct me if I am wrong.
2. My cationic polymer has a lot of primary amine and imine groups, and I plan to use Chondroitin sulfate (sulfated GAG) as an anionic polymer. What should be the best pH value for my system, considering highly acidic conditions are not good for MnCO3.
3. What is the optimal concentration value for polymer solution? I used 1 mg/mL. What should be the v/v or w/v ratio of particles and polymeric solution.
4. How to and for how long should i disperse particles in polymer solution? Do i need continuous stirring? or just dispersion for some time in polymer solution without continuous sonication or stirring?
5. particle in polymer solution takes ages to settle, can i use centrifugation? will it have any effect on polymer coating? some paper suggest it will damage coating.
6. how to analyze the efficacy of coating?
Zeeshan Uddin
1. MnCO3 is hydrolyzed by cation and anion. Look at the dissociation constants of carbonic acid and manganese hydroxide in the tables and draw your own conclusions.
2. For a cationic polymer, the particle charge should be negative. Determine the charges of nanoparticles using the DLS method.
3. Use 1mg / L
4. Stir without ultrasound. The local pressure created breaks the polymer chains.
5. Use centrifugation to wash off excess polymer.
6. See my answer to your other question.
Yuri Mirgorod sir, thanks for your suggestion.
To my surprise and published results (+30 mv), the zeta potential of MnCO3 is about -10.0 mv in PBS. so I will start with cationic polymer.
can I use zwitterionic polymer for coating?
The charge of the zwitterionic polymer depends on pH. Therefore, decide for yourself.
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