Hi all,
I plan to do IHC to distinguish monocyte-derived macrophages from interstitial and alveolar macrophages in the lung in non-human primates, but I'm having a hard time finding a good marker. It seems that I can distinguish alveolar vs interstitial macrophages with CD206 and CD163, then maybe use CD14 for monocyte-derived macrophages, however, I'm seeing literature describing the loss of CD14 and gain of CD206 in monocyte-derived macrophages that have remained in the lung and begin to undergo "polarization" as replacement alveolar macrophages after infection. Anyone have suggestions or suggested reading?
Hello,
I have some experience on murine alveolar macrophages. I was checking on some literatures on human alveolar macrophages and found the following article(take a look at Table6); SP-D may be a good candidate.
Hope this helps.
Article Immunophenotyping of macrophages in human pulmonary tubercul...
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