How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Does anybody know the ways of homogeneous ripening of Mung bean crop?

Some crops (Mung bean) are matured at different times i.e., harvested at several times that's why the labour cost of the farmer is high. So, could you please, is there any ways or chemicals are...

12 February 2021 294 4 View

Why whitefly prefer not to infest Pigeonpea?

Whitefly, Bemisia tabaci is pest of broad host range. In pulse crops, it generally infests greengram, blackgram and cowpea but not Pigeonpea. There might be some biochemical or morphological...

09 November 2020 9,199 3 View

Is any research paper available on bioinsecticidal properties of some plant extracts on Cowpea weevil (Callosobruchus maculatus)?

We want to see the bioinsecticidal properties of some plant extracts on Cowpea weevil (Callosobruchus maculatus) on stored Cowpea Vigna unguiculata.

13 February 2020 8,987 8 View

Is there a working protocol for microtomy of Vigna radiata root samples?

A simple and cost-effective protocol for sectioning of Vigna radiata root samples.

02 February 2020 786 1 View

Is any research paper available on bioinsecticidal properties of some plant extracts on Cowpea weevil (Callosobruchus maculatus)?

We want to see the bioinsecticidal properties of some plant extracts on Cowpea weevil (Callosobruchus maculatus) on stored Cowpea Vigna unguiculata.

01 February 2020 5,185 6 View

What is the protocol for sectioning of roots of Vigna radiata for SEM Analysis?

My team wants to study the cell wall architecture of Vigna radiata roots by using SEM. Is there a clear protocol for preparing root sections for SEM Analysis?

08 January 2020 6,527 3 View

Primers forward and reverse for the molecular marker named MahSe2 ?

Hi all I am working on markers assisted selection and I wish to know the sequence of primer of this marker(MahSe2) linked to resistance of cowpea to Striga. Best regards

06 January 2020 9,978 4 View

Primers forward and reverse for the molecular marker named MahSe2 ?

Hi all I am working on markers assisted selection and I wish to know the sequence of primer of this marker(MahSe2) linked to resistance of cowpea to Striga. Best regards

31 December 2019 3,138 4 View

What could be the band on the lower portion of the gel image?

I have isolated mung bean's DNA using Qiagen DNA isolation kit from dried leaves of this plant. But I have obtained an additional low molecular weight band which I presumed to be of RNA but still...

10 November 2019 4,860 2 View

How to proceed for DNA purification ?

Hi everyone, I am working in cowpea genetic diversity and I have an issue with DNA extraction, I always get a bad quality of DNA with a lot of contamination.I am looking for a best protocol to...

08 September 2019 2,293 4 View