I have finished quantifying results for pollen viability using a staining method (Alexander Dye) coupled with a haemocytometer. Following this I wish to perform analysis of MDA and proline content in my floral material. I also hope to perform molecular analysis on my floral material, as another avenue to investigate pollen viability.
I am just curious as to which genes of interest others have investigated when analyzing the link between the genes involved in pollen viability and heat stress exposure. Any information will be greatly appreciated in restricting my literature search.
Thank you
Dear Nicholas
High temperature leads to a decrease of pollen viability which is directly correlated with a loss of fruit production. The reduction in pollen viability is associated with changes in the level and composition of several (groups of) metabolites, which play an important role in pollen development, for example by contributing to pollen nutrition or by providing protection to environmental stresses. This review will give an overview of the current state of the art on the role of various pollen metabolites in pollen homeostasis and thermo-tolerance. Their possible use as metabolic markers to assist breeding programs for plant thermo-tolerance will be discussed.
Dear Seied,
Thank you for your feedback and the attached research paper. It is very much appreciated.
Kind Regards,
Nicholas
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