Interdendritic phase coarsens during homogenization?

Hello Is it possible for the micro-segregation (non-equilibrium phases) coarsen during the homogenization treatment? There is an obvious reduction in the quantity, but some of the phases remained...

19 September 2020 4,985 3 View

Preparing ethanol standards for HPLC?

I need to prepare ethanol standards for HPLC to quantify the bio-ethanol in my sample of interest. What range of known concentrations would I have to prepare for estimating ethanol concentration...

15 October 2019 8,844 5 View

Quantitative data from a regular FT-IR?

Hi, ATR-FT-IR spectroscopy is being used for the quantification of a particular compound. Is it not possible to run a known quantity of a sample in a regular FT-IR and then compare the peaks...

22 August 2019 9,552 17 View

Can anyone help me with an SDS PAGE's image?

Hi, One of my colleagues has used a pre-cast, commercially available 10% separating gel for his SDS PAGE. Later, while interpreting the results, we observed a broadening of the marker lane...

14 August 2019 8,943 17 View

Grid size in material diffusion simulation - Physical significance?

10 October 2018 8,213 1 View

Is it necessary to prepare all the biological replicates on the same day?

I am wondering whether it is completely necessary to have a biological replicates for an RNA seq experiment to be prepared on the same day. What if in case of a differential gene expression...

20 September 2018 1,825 5 View

Chemical mobility from Darken's equation?

19 June 2018 8,585 3 View

Why 002 reflections are absent in spinel oxides (FCC)?

For example, magnetite (Fe3O4) does not contain 002 reflections but contains 004 reflections, though it has FCC structure. Is it because of the structure factor? In my case, I have an oxide that...

29 April 2018 2,053 4 View

Can i use anastrazole steroid used by bodybuiders to study its effect in rat breast cancer model?

I have to give oral dosage of anastrazole for three months.

29 December 2017 4,977 1 View

What is the difference between graphene and carbon quantum dots??

both quantum dots have the same graphite structure with lattice spacing around 0.34 nm. how can we differentiate?

01 October 2017 3,107 7 View

How can i resolve the "final solutuin is not converged" error in Comsol for my heat transfer simulation??

I have been trying to simulate heat transfer problem in Comsol 5.5 for long time but it always throw an error stating: "Failed to find a solution. Maximum number of Newton iterations...

28 February 2021 8,626 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

How to approach CFD simulation results of a flow past a cylinder?

Hi all, I'm doing a transient simulation of flow past a rectangular cylinder close to the free surface. As far as I'm concerned, the common practice is to discard the initial stages of the...

25 February 2021 1,348 3 View

Error in comsol?

Dear respected researchers, while performing CFD analysis in Comsol it's showing like this Error: Nonlinear solver did not converge. The maximum number of Newton iterations reached can...

24 February 2021 249 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

How can I get the coding gene sequences of Hela and U2OS cell lines? Is mRNA sequence of Hsp90 alpha tissue specific??

I am looking for hsp90 mRNA sequence in Hela and U2OS cell lines, I have tried through ncbi but always the output is hsp90 in homo sapiens not in the specific cell line. Kindly, could any one...

22 February 2021 1,853 3 View

How to use Remote sensing and GIS applications for mapping the available medicinal plants within the locality/municipality?

Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...

21 February 2021 374 5 View