Hi everyone,
I would like to test the effect of some microalgae (as a source of bioactive compounds) on lipid accumulation in 3T3-L1 adipocytes.
My concern is how handle microalgae and treat cells; due to their characteristics, specially as unicelular organisms, microalgae can be added to the incubation media of cells, and it seems that there are not afected after 24 hour treatment with a high dose. However, if there is microalgae cell wall all the bioavtive compounds remain inside them, is ¡n´t it?
I would be very gratfull if you could recommend me if it is better to make a cell disruption and ad this to the incubation media,
or
If it could be better to try an extraction with solvents.
It´s my first time working with microalgae in cell culture, could you help me? any specific protocol?
Thank you in advance
The treatment is mention in my research read it how do cell determine its size to grow
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