Dear Mikhail,

we used only dry biomass for extraction.

please follow the given links which will help you.. one of the article is a review article on lipid extraction from microalgae so you will get good information regarding your doubt.  

with regards,

Perumal

https://www.researchgate.net/file.PostFileLoader.html?id=561378f85dbbbd41bd8b4665&assetKey=AS%3A281460104810510%401444116727066

http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0089643

https://www.google.co.in/url?sa=t&rct=j&q=&esrc=s&source=web&cd=5&cad=rja&uact=8&ved=0ahUKEwidlcSTucLOAhVLtY8KHfZMBu0QFghBMAQ&url=http%3A%2F%2Fwww.mdpi.com%2F1660-3397%2F13%2F8%2F5111%2Fpdf&usg=AFQjCNFMvb7NfmaVlYBm9XWx-nzexpLvpg&sig2=otXJTvMAF2Dttj58ypfNWQ

Thank you very much for your help, Elaya Perumal Ulagalanthaperumal !

We used following method in "1Yazıcı Z., Aysel V., Öksüz E., Köse A., Cumalı S., Güven K.C. Fatty acid composition of marine macroalgae from the Black Sea and Dardanelles. Toxicological & Environmental Chemistry, 89(2): 371-379, 2007. DOI:10.1080/02772240601012366)

Freshly collected algae were thoroughly cleaned with seawater followed by running tap water to remove adhering particulate matter and epiphytes. The material was dried to constant weight and then pulverised. Accurately weighted portion of each sample was homogenised in cold 154mM NaCl. Total lipids and added internal standard (100 mg nonadecanoic acid in chloroform, Sigma Chemical Co., St Louis) were extracted with chloroform/methanol (2 : 1). The chloroform phase was removed and evaporated to dryness under a stream of nitrogen. Total lipids were saponified with 2% KOH in methanol and the FAs methylated with 14% BF3 in methanol. The resulting FA methyl esters (FAMEs) were extracted with hexane and analysed by capillary gas chromatography.

Thank you very much for your help, Abd El-Fatah Abomohra!

Thank you very much for your help, Zeliha Yazici!

dry algae powder is preferred, however, if you will apply a method like Blight & Dayer, 1959, you can use algal paste.

Thank you, Nagwa Mohammady!

I usually start from frozen pellet, remove all water, and then continue with a single step extraction as described in this paper (see attachment). But I know you can start from dry or lyophilized biomass as well.

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0089643

Thank you very much, Lorenza!