Hi,
i am performing TLC for seperation of alkaloids from the extract..But, alkaloid of my interest was run along with mobile phase and resolution is also poor , hence creating problem in its isolation.
What can i do to obtain better results?
I have used CHCl3:Ethyl acetate:methanol(4:5:0.5), it gives poor results...Currently , i m using Chloroform:Methanol (24:1).
Pl you try Toluene:EtOAc:MeOH:ammonia solution in the ratio of 40:40:7 to 9 ml: 2-4 drop ammonia solution. I hope you will get good separation. Still you have any problem you can consult Plant drug analysis book.
Pl you try Toluene:EtOAc:MeOH:ammonia solution in the ratio of 40:40:7 to 9 ml: 2-4 drop ammonia solution. I hope you will get good separation. Still you have any problem you can consult Plant drug analysis book.
Thank you sir,.......this mobile phase really give good results....!!!!!!!!!! Thanks.....
Ajay, your mobile phase worked for ramana. What may be the reason behind this as he was already using a mobile very similar to one you suggested except for ammonia. would you explain please.
Alkaloids are basic in nature. For the separation of alkaloid basic medium is needed for good resolution. Like in HPLC basic buffer required for the separation of basic compounds and acid buffer required for acidic compounds.
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