Hello Everyone,
I need to determine the enzyme activity for two commercially bought enzymes (Cholesterol Esterase and Butyrylcholinesterase) before I begin my research project. Does anyone know of a kit, protocol, or cited sources that I could use? Thank you in advance for you advice.
Dear Shelley,
For the determination of the activity of Cholesterol Esterase, please see the paper contained in the following link:
http://www.actabp.pl/pdf/3_2013/401.pdf
For the determination of the activity of Butyrylcholinesterase, please see the paper contained in the following link:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4596826/
Hoping this will be helpful,
Rafik
Thank you so much Rafik! This is exactly what I needed. I really appreciate your help.
Ellman is a common methd for determination of cholinesterase activity.
Next to that, I'd assume that the information sheet for your commercial enzyme includes some mention of its activity. For instance, on the BuChE from Sigma Aldrich, you get:
"Specific activity: ≥ 900 units/mg protein
Unit definition: One unit will hydrolyze 1.0 μmole of butyrylcholine to choline and butyrate per minute at pH8 at 37°C. "
So, if you want to check the activity of your enzyme, you need to check on the same substrate and see if the values you get for this substrate are in line with specs. Or, would you like to check the activity of those enzymes against a new substrate? (e.g. your drug candidate?)
please see the paper given here.
https://www.researchgate.net/publication/280602851_A_cold-adapted_solvent_and_salt_tolerant_esterase_from_marine_bacterium_Psychrobacter_pacificensis
Regards
Article A cold-adapted, solvent and salt tolerant esterase from mari...
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