Is it possible to predict the conformations of the molecule in the solution based on quantum-chemical calculations?

Hello, everyone and thanks for helping me. My question is: I have a metal complex that has several possible conformations. I need to calculate what conformation is the most plausible in different...

08 January 2021 831 3 View

HFSS / Radiation efficiency?

Hi! I'm new with HFSS. Could somebody tell how HFSS calculate radiation efficiency? In my project I have small value of reflection coefficient at the antenna input (s11 = - 15 dB), but difference...

26 April 2019 4,149 18 View

How can I measure the binding energy experimentally?

I have a wild-type enzyme 'Ewt' and few mutant forms of this enzyme ('Emut1-Emutn'), and I want to compare energy of binding (Gb) of a substrate 'S' to these enzymes. I know that binding energy...

04 October 2015 2,938 9 View

Can someone give advice on how to clear my confusion with IgE-binding sites prediction?

Dear colleagues, Currently I'm trying to predict probable IgE-binding regions in one of the allergenic proteins and reveal candidate residues for mutation in order to design a...

08 February 2015 429 1 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

HPLC baseline failure (ACN/Water)?

My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...

21 February 2021 5,656 3 View

How to get/count refractive index in gasses?

20 February 2021 8,202 6 View

Which transfection reagent is the best for plasmid transfection of MDA-MB-231 cell line, Lipofectamine based or other reagent suggestion?

I try to transfect MDA MB 231 cell line with a plasmid by PEI, unfortunately, the rate of transfection is low, and now I wanna find and order the best transfection reagent for this cell line and...

19 February 2021 828 6 View

Any information about dinitrosalisylic acid reagent end point color stability ?

I work on estimation of alpha amylase activity using dinitrosalisylic acid reagent If i put the reagent to stope the enzymatic reaction and allow the reagent to react with the produced reducing...

17 February 2021 7,402 4 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

How to inerpret whether PCR went OK?

Dear friends! I am back to work after 3 years career break. Now performing a PCR, feeling like a newbie... Please, help to understand whether RT-PCR reaction "worked" or not. Which results should...

14 February 2021 3,129 8 View

Can any one explain why the ethyl ester Biodiesel (FAEE) has higher than that of the metyl ester FAME- both of them are derived from the same oil?

Biodiesels derived from waste vegetable oil transesterification with Ethanol (FAEE) show higher peroxide values than those derived by transesterification with methanol (FAME) prepared under the...

13 February 2021 193 6 View