Greetings to all,
Does anybody know where I can find membranes with a mesh size that fungi could not be pass ?
In my experimental system I need to grow a fungal colony in a "Tea bag" and avoid any fungal growth out of this tea bag compartment, I only want to have full exchange of nutrients and other compounds between the fungus containing compartment and the rest of the media.
Did anyone try something similar ? any recommendation for a company which from which I can order such membranes ?
I appreciate your help and time,
Considering the size of fungal hyphae, you will need to use a membrane with ≤ 5 µm mesh size. Free movement of liquids will be impaired somehow as you can imagine but I think diffusion should be fine. Whether you will have full exchange of nutrients and other compounds between the fungal containing compartment and the rest of the media, am not sure. Try Sefar AG (http://www.sefar.com) for the membrane.
Thank u Benard, I had a look and I found many products, none of which are in the ( Cloth) form which can be made as a tea bag .. I will keep looking around .
Ashaimaa, thank you for the suggestion, I checked the product u recommended but unfortunately it has a big mesh size "22-25 µm". This will not filter the spores and the hyphae .. I am checking the website to see if they have different sizes ..
I appreciate any further recommendation
You can use discs of sterile cellophane paper. It can be deposited in Petri dish with water and sterilized at 121ºC and 15 psi. I have growth R. solani on that paper
Thank you Mr. Cedeño,
Unfortunately, my fungi degrade cellophane, that is why I was looking for alternatives.
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