I am trying to express streptavidin with periplasmic leader sequence in mycobacterium smegmatis but the protein is not expressing?

15 September 2023 0 2K Report

I tried to do same with membrane fraction. It expressed in membrane but somehow not working in periplasm

Badges
Science topic

More Neha Quadir's questions See All

How can I precipitate a protein from condition media ?

So I want to see is the level of a secreted protein in the condition media via western blotting What my doubt is 1. How much cells should I seed and what should be the volume of condition...

19 June 2024 1,927 1 View

By mistake my friend dipped Ag/AgCl reference electrode in KI instead of KCl now is is not working then what should we do to save the electrode?

this electrode can't be open.

13 May 2024 2,991 3 View

FAD containing Protein purification?

I am trying to express a FAD containing enzyme of mycobacteria in E.coli. I am able to purify the protein which is slightly yellow in color but it seems that my FAD is all unbound to the protein....

16 April 2024 6,912 1 View

How to make reduced form of NADPH?

I want to prepare reduced form of NADPH. I am using 100mM of Tris (pH8.0) to make the buffer but the absorbance is showing to be 1.6 at 340nm which should supposed to be 0.6. can someone please...

11 March 2024 4,629 3 View

What is an article?

need a. Suggestion for best article related to management studies in data science

07 March 2024 3,506 1 View

Are there specific biochemical tests designed to analyze the ADMET properties of a particular compound?

Are there specific biochemical tests designed to analyze the ADMET properties of compounds, providing insights into their Absorption, Distribution, Metabolism, Excretion, and Toxicity?

25 December 2023 4,699 2 View

Anyone needs guidance on mendeley reference manager? Are you researcher, student or author?

If you want to save time on manual referencing, I have posted 2 hr video on udemy. Mendeley desktop and web version helps us to store, organise and use reference as per any reference style. You...

28 September 2023 5,418 2 View

Hi, I'm facing issues in synthesising a chalcone using acetophenone and aldehyde with hydroxy substituent. Any tips on how to carry out the reaction?

My reactants are not consumed and no new product spot is formed. My reaction conditions include dissolution of the acetophenone and aldehyde derivative in ethanol followed by dropwise addition of...

24 September 2023 1,908 5 View

If I have to heat my plant protein isolate to get better digestibility. What are the best conditions for the heating (Time and temperature). ?

To get better digestibility of the plant proteins (Fava bean protein isolates). What heat treatment should I give before the IVD (In vitro digestion) process ?

20 September 2023 7,124 0 View

Can eudragitE100 be used for delivery of a hydrophilic drug?

17 September 2023 4,135 0 View

What is the reason I get 400nm nanoparticles using an extruder with 200 nm membrane for cell membrane coating?

I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...

30 July 2024 3,294 3 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

Does it exist some special technique for cutting ceramic membranes for Cross-sectional FESEM images?

Does it exist some special technique for cutting ceramic membranes for Cross-sectional FESEM or SEM images?

25 July 2024 5,482 2 View

Issues with Malaria Coated Nitrocellulose Membrane Releasing Moisture?

Hello, I am currently working with Malaria Pf/Pan nitrocellulose membranes coated for malaria detection. After drying the coated membranes in an Incubator (overnight at 37°C), I've noticed that...

16 July 2024 7,937 1 View

ELISA sandwich with two monoclonal antibodies ?

hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...

16 July 2024 9,044 10 View

How can we test a Nafion membrane ?

we have a nafion 117 membrane and we want to know if it has the properties claimed by the sellers. what are the tests available ?

13 July 2024 503 1 View

How to wash capsule filters (Membrane: polyethersulfone; pore size0.2u/0.45u) after filtration. can I wash by NaOH? if yes, what will be the molarity?

11 July 2024 8,969 1 View

How can I solve this problem with my western blot?

I have this problem with my western blot for the ABCG2 antibody on nitrocellulose membrane. Help please

02 July 2024 8,715 2 View

How to insert cholesterol inside DPPC membrane by replacing same number of DPPC molecules in GROMACS?

I am trying to insert cholesterol inside the DPPC membrane by using gmx insert_molecules -replace. But number of DPPC molecules replaced is much higher than the number of cholesterol inserted?...

30 June 2024 4,160 2 View

How to assess bacterial permeability?

Is there an established protocol to measure the permeability of bacterial membranes? Thank you

30 June 2024 6,614 3 View