Greeting to all,
I have modelled two different domains of the same protein from two different tools (example - 1-250 using ab initio and 250-400 a.a using modeller). Now to make it a full length protein structure I want to fuse this model and I have tried "Join Models" of UCSF chimera for the same purpose. But after fusion of the two pdb structures with the above tool, the quality of the structure is getting reduced even after refinement. what should I need to do to correct the fused model.
Note - Quality of the individual models before merging was fine.
Thank you in advance.
This type of query is already answered at the link
https://www.researchgate.net/post/how_to_merge_protein_fragments_into_a_full-length_structure
You can try the AIDA webserver to model the full length structure based on the two individual structures : http://aida.godziklab.org
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