Melt flow rate, melt viscosity and intrinsic viscosity relationship?

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How can I differentiate full structural equation model (measurement model + structural model) from (just) path analysis?

Even in some high impact journals, there are articles in which - there is no description that a variable is a latent (unobserved) one, - the researcher(s) do(es) not report the statistical method...

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Cell numbers in powder increase again after decreasing?

I used the raw substrates for formulation of bacteria for biocontrol. During storage, cell numbers decreased continuously during the first two months. However, it increased again after 2 months?...

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Does someone know how to co-stain foxp3 and phospho-protein for flow cytometry analysis?

Now I'm trying to analyze phosphorylation of signal molecules in Treg. To do that, initially I chose foxp3 staining kit from e-bioscience. But most of cytosolic proteins were gone, although foxp3...

26 August 2016 2,244 12 View

What kind of ANGPTL4 antibody is suitable for Apoptome?

I have to work on ANGPTL4 expression in lung cancer by using Apoptome immunofluoresence microscope. I have no experience in IF and I have difficult in choosing primary and secondary antibodies...

15 June 2016 5,793 1 View

How shall I develop measurement items for an acceptance study using Technology Acceptance Model?

Most of the technology acceptance studies adapt measurement items from previous validated questionnaires. How shall I develop a measurement for a latent construct which has almost never been...

13 October 2015 1,314 7 View

What is the best diagnosis for these sections of liver ?

hello every one, can any one ANNOTATE these sections ?? with best histopathological diagnosis ? I have some notes but i want the best annotations, Because i am new in this disciplinary !

01 April 2015 2,871 6 View

How do you generate a dendrogram from repetitive element PCR?

I would like to know how to generate a dendrogram from repetitive element PCR profile. Please see the attached publication. If somebody knows how to do that, please teach me.

22 August 2014 9,796 3 View

Is there any acceptable R2 value for educational research in PLS-SEM?

I am doing PLS-SEM.According to Joseph F. Hair and his colleagues, the acceptable level of R2 depends on the research context with higher values indicate higher level of predictive accuracy. In...

01 January 1970 8,531 4 View

What should I do if high cross loadings between PU and BI indicators in TAM?

I did SEM for my TAM model and found high cross loadings between PU and BI indicators. However, factor loadings on respective constructs are higher than cross loadings and Fornell-Larker criterion...

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Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Modeling viscosity using model of Chung?

Hello, As part of simulation of gases mixture and water, I need to calculate viscosity of the fluid components and I am using relationships proposed by Chung et al. (1988). The irony is while I...

25 February 2021 8,053 5 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

How to determine number concentration of polymer solutions using viscosity experiment?

I have synthesized PNIPAM (Poly (N-isopropylacrylamide)) microgels, which are copolymerized with oligo-ethylene glycol based comonomers. I have to determine the exact number concentration for each...

19 February 2021 5,205 5 View

Dealing with BMDM seeding and RNA extraction ?

biomedical master student here , i am dealing with mice BMDM and would like some help , i seed 0.5 x 10^6 cells/per well in a 6 well plate but when i extract the RNA i get a very low yeild , i...

15 February 2021 1,102 5 View

How long can agarose embedded tissues be kept?

I am embedding PFA fixed tissues in low melting point agarose prior to sectioning and I am wondering how long can these can be kept whilst maintaining tissue integrity and fluorescent signal?...

14 February 2021 6,688 3 View

What is a suitable method to freeze feeders-free, serum free episomal hiPSC?

Hi everyone, I need your professional advice: I started growing episomal iPSC (commercial cell line). I grow them in StemFlex medium. What would you recommend as the most effective freezing...

07 February 2021 8,591 4 View

Simulation of melting process in CFD ?

Hi everyone, I'd like to know if Polyflow software can be used for the simulation of melting process of polymers. I thought to model solid phase as a fluid with very high viscosity, and use...

03 February 2021 8,109 3 View

Freeze/Thaw of LS174t cells?

Hello World, I have been working with LS174t. Complete Growth Media- 10% FBS/EMEM. ATCC says to freeze with 5% DMSO/CGM. when I defrost my vials, they are not attaching to flask. vials put into...

01 February 2021 8,723 2 View