How could I determine the starting methyltransferase enzyme and methyl donor to methylate a synthetic peptide?

Hello, I have a synthetic peptide with arginine residue I aim to methylate using PRMT1 + SAM (methyl donor). I then will use my dimethylated arginine peptide product in ELISAs and Western blots....

12 November 2020 5,568 7 View

ELISA-Why does my diluted high absorbance sample have such lower concentration compared to less diluted sample when using standard curve?

Hello! Our lab has developed a standard curve with known concentration that we use to determine our ELISA concentrations. We then multiply by the dilution factor for our final concentration....

25 October 2020 8,601 6 View

How to handle the analysis of yield stability or yield risk based on simulated crop yield data?

Dear everyone, do you have any experience on the analysis of yield stability or yield risk based on simulated crop yield data? I would like to use a modelling approach and simulate grain yields...

23 February 2020 1,584 6 View

What is the best way to calculate the yield of an entire crop rotation including different kind of crops ?

What is the best way to calculate the yield of an entire crop rotation including different kind of crops and different yield levels ? For example the following cropping sequences: 1. sugar beet...

03 December 2019 9,527 11 View

Protocol for whole heart tissue lysis for Westerns?

Hello, I am troubleshooting a western protocol using whole heart homogenates but I seem to be getting consistent smearing at the lower band range of my gel which is also where I want to see my...

01 October 2019 8,682 4 View

What is the minimum sample size for factor analysis?

I will be doing ryff's psychological well-being scale for psychometric properties. What would be the minimum sample size for factor analysis using spss? I read Slovin's formula is not reliable?

26 April 2017 601 7 View

Could alternate form reliability be administered simultaneously?

I will be doing ryff's psychological well being scale's psychometric properties. Could I administer alternate form reliability simultaneously? (42-item and 18-item)

25 April 2017 8,184 3 View

IS Confrimatory Factor Analysis CFA and WLS enough for psychometric evaluation?

Im going to do a psychometric evaluation on ryff's scale using nurses as my sample. Would CFA and WLS suffice? Thank you!

13 April 2017 4,938 3 View

Can Zung's Depression Scale be administered by a student?

I am currently doing my psychometric evaluation on ryff's scale and would like to use Zung's scale for my divergent validity. I am an undergrad student. Can I administer it?

09 April 2017 510 5 View

What would be a good discriminant validity for Ryff's PWB?

04 April 2017 5,633 3 View

How to compute the internal consistency of a scale with ordinal items?

Hi community, I am trying to determine what is the best way to calculate the internal consistency of a newly developed self-esteem scale. I am working together with two other researchers on the...

28 February 2021 1,369 5 View

How can I set up a good fit SEM model in AMOS?

I am trying to make an SEM model in AMOS for my dissertation but having some trouble with getting a good fit. I am looking for relationships between self-efficacy reported on a Likert scale and...

26 February 2021 8,642 4 View

Inpatient DBT to reduce parasuicidal behaviour?

Currently trying to obtain as many pieces of research as possible for my dissertation around the implementation of DBT within the inpatient unit for those with a diagnosis of BPD. Primarily...

23 February 2021 5,654 2 View

Latent profile analysis based on two variables/sources of data?

Hello, for my master project, I would like to conduct a latent profile analysis about self-regulated learning skills. I would like to take two different measures into account when creating the...

21 February 2021 2,326 3 View

What are some alternatives to the Self-Directed Learning Readiness Scale (SDLRS) developed by Dr. Lucy L. Guglielmino?

The Self-Directed Learning Readiness Scale (SDLRS), also known as the Learning Preference Assessment (LPA), was developed by Dr. Lucy M. Guglielmino in 1977 to measure learners' attitudes of and...

15 February 2021 3,095 4 View

Do you know of an informal caregiver of a person who has recently received a bone marrow transplant?

We are conducting a pilot study of an intervention for informal caregivers of persons who have recently received a bone marrow transplant in the US. If you know of anyone who might be interested,...

13 February 2021 9,174 1 View

Seeds harvest from white clover by selfing?

I tried to get seeds from white clover by self-fertilization but I could not get the seeds. As you know the white clover is generally fertilized by crossing. I wanna get the seeds by...

04 February 2021 9,152 4 View

Identical spectra for L- and D-peptides after they self-assemble?

Hi dear all, I need advice. I have two peptides with the same sequence but one consists of L-amino acids only and the other consists of D-amino acids only. when they self-assemble in aqueous...

03 February 2021 2,032 3 View

Whether L version and D version peptidesshould have identical NMR, especially in the state of self-assembly?

Whether L version and D version peptides should have identical NMR, especially in the state of self-assembly have to have identical NMR? How to use HRMS to confirm L version or D version...

01 February 2021 6,756 4 View

Any suggestion on self-efficacy scale ?

Dear researchers, I am planning on conducting a research on the relationship between Language learning strategies, by Oxford (1990) and self-efficacy. I have found Morgan-Jinks Student Efficacy...

22 January 2021 8,432 3 View