Protocol refs appreciated
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23 July 2023 5,075 1 View
i could not find out JCPDF card for XRD results of this compound in xpert high score. how can I interprete this?
18 May 2023 8,293 2 View
I am working on Thalassemia patients and want to check their hypoxia status by expression analysis of the concerned genes. Could anyone recommend which gene would be more suitable in this case;...
30 January 2020 8,365 3 View
I am extracting Lipids from RBC Membranes using the Cholorform/Methanol (2:1) method. After phase separation and consequent evaporation of the organic phase, what would be the best way to confirm...
02 December 2019 8,169 2 View
I am working on hypoxia induced lipidomic changes in leukemia cell line. I induce hypoxia by CoCl2. I want to know if their is an easier method (some kind of dye or a distinct morphological...
15 October 2019 7,740 3 View
Please recommend some paper with precise calculated recoveries.
11 October 2019 4,103 0 View
I want to know the protocol for Isolating RBCs from whole blood collected in EDTA tubes. I am planning on studying the lipidomics of the RBC Membranes therefore need to isolate RBCs in intact form.
23 November 2018 9,157 3 View
How long RBC can remain intact in whole blood collected in EDTA Tubes before being processed for Isolation of RBCs? I want to know conditions for storage of such samples so that they can be...
23 November 2018 9,482 14 View
Can anyone suggest me about the flourcent primers for Aflp analysis?. FAM is the common flourcent dye which is used in one of the selective primers of the AfLp, I want to lable my ECOR1 primer...
31 October 2018 1,748 5 View
Greetings I am doing Aflp, for my pre selective amplification step i am using two primers Mse1+1 base and Ecor1+1 base. But both the priners have different annealing temp 52 and 55. I tried...
25 October 2018 2,946 3 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...
06 August 2024 4,953 0 View
Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?
06 August 2024 980 5 View
There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
05 August 2024 4,945 3 View
Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?
03 August 2024 7,035 3 View
or we can collaborate
01 August 2024 6,705 2 View
Hi everyone, Recently I have been conducting Dynamic Light scattering experiments in a micellar solution at 5 and gel at 37 degrees of Celsius with latex particles of diameter 190-500 nm. While...
01 August 2024 1,168 4 View