Is there a universal method to measure the cholesterol content of biliary and fecal samples in mammals? I'm looking for a method to estimate total cholesterol content and that doesn't require a HPLC machine.
Hi,
you can use a free cholesterol assay kit http://www.sigmaaldrich.com/catalog/product/sigma/mak043?lang=fr®ion=FR&gclid=CIuOus_Q5tACFRUTGwodPB4Lkg
rgds
Hi!
You could extract the lipids (by using the Folch or Bligh and Dyer methods, google will find these easily) and then use thin layer chromatography to separate free cholesterol as well as cholesterol esters from other lipids. These methods will require solvents (MeOH, chloroform, probably ether for the TLC running liquid), a stream of nitrogen (for evaporation of lipids without oxidizing them) and a thin layer chromatography running chamber, but not HPLC machine. These methods will probably give you more reliable results than any available kits, but do require some time to set up and may not be the best option if you only want rough estimates of cholesterol levels.
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