I would like to measure the nitrogen rate of dry plant sample by kjedhal method, and I don't know if it is possible to make just the first stage ( the mineralization) and Keep the mineralized sample for a while before finishing the other stages (distillation and titration).
an other question , I would like to replace the titration step by the Nessler method ( colorimetric measure) , please can you tell me if the distillation step is necessary .
thank you for your response.
Hello Dahmani,
Regarding your first question: you can keep the mineralized samples for any time before analysis; HOWEVER, make sure they are well closed as atmospheric ammonia readily dissolves in acids and can cause significant contamination.
I do not know about the second question. I and recommend automated flow injection analysis (FIA) in an analytical lab as this method is easy, fast and inexpensive.
Yes, as suggested by Dr. Tomas, you can keep and use later your samples to analysis.
There are several available methods,
The cumulative nitrogen intake (rather than uptake) and then infer a rate. For this, you need, at 2 sampling times, the knowledge of the whole plant (or stand) dry biomass and mean tissue. In the open field, this is a classic method. However, if your experimental facility has a restricted area, it may be unworkable to sample whole plants along a year (furthermore, border effects are extremely high in terms of plant biomass and , and the harvest of a plant influences greatly the neighbours). You could envisage the subsampling of plant organs (leaves, stems & roots), but you have to know that [N] varies greatly with the organ's age, and exposure to
https://www.researchgate.net/post/Can_anyone_suggest_a_procedure_for_determining_nitrogen_uptake_in_the_plant_roots?_tpcectx=profile_questions
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