Why qPCR is showing such curve instead of an perfect one on the following picture ?

Dear all, I wonder if you could help me with the interpretation of such charts I get sometimes when I do amplification of covid19 by using ORF gene ( please see the picture i attached the graph...

07 June 2020 7,656 3 View

How to measure accurate specific heat capacity with DSC?

I am using Mettler Toledo DSC 2 to find cp of In/Zn with sapphire and direct methods. The DSC is calibrated with both In and Zn and is within the specifications. However when I try to find cp...

16 September 2019 9,331 8 View

Please guide, which gene primers are used for quantification analysis of cell free and circulating tumor DNA gene ?

I am to find out the quantification of circulating tumor DNA and cell free DNA through real time PCR, for that purpose in PCR which gene primers should be included for amplification

28 August 2019 5,538 4 View

What is the difference between Group of Pictures (GOP) and video?

I am currently working on video transcoding. I was going through literature and I saw that the authors of that literature have considered group of pictures for transcoding rather than video. Is...

21 August 2018 6,868 3 View

Possibility to get sequence information of primers premixed in a ready to use PCR kit/master mix?

03 September 2017 1,559 3 View

Expression of penta-peptide in a suitable Mammalian expression vector?

Hi I need to express a 5 AA long peptide in any suitable mammalian expression vector. CI fear that if its allowed to be expressed in pCDNA3.1, there might be a leaky overhangs of extra amino acids...

21 March 2017 7,432 3 View

Future of electronics will soon depend on graphene nanoribbons. Anyone know how to control the ribbons growth and their alignment?

Graphene nano-ribbons can be grown directly on the surface of a semiconductor, but it's hard to control the ribbons growth, and at the same time, aligning them all in the same direction.

06 February 2016 144 2 View

Does anybody have experience in gene sequencing through HRM?

A.o.A , Please let me know if any one has used High Resolution melting analysis technique for mutation analysis. If yes than please which type of Real Time PCR is used. What other accessories...

01 February 2015 1,635 1 View

How can I perform Search and Filtration Funcationalities on Database with some Knowledgebase?

I have a database with millions of records stored in it. I want to implement Search and Functionalists in My db with some AI and data mining techniques and methods. Suggest me Some Algo, Methods...

04 December 2014 7,605 2 View

Is there a good antibody available to detect C-terminal fragments of Amyloid Precursor Protein (695 AA isoform)?

I am trying to quantify the levels of C-terminal fragments of APP, generated after BACE1 cleavage in APP/swAPP cell lines (in different experimental conditions). I have tried some commercially...

26 June 2014 5,572 7 View

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...

01 March 2021 2,327 1 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

How to prevent ANFIS Model from negative prediction values in MATLAB?

I have input and output data set for "ANFIS modeling in MATLAB", and I am getting some negative predicted values of output in testing. However, the predicted values of output in training are...

28 February 2021 3,459 3 View

How does an AC-DC power supply Flyback Converter Based USB Charger Model using Matlab/Simulink looks like?

I am required to learn about Flyback converters and I got stuck not knowing to full design of the power supply flyback converter Based USB Charger Model using Simulink, especially the design model...

25 February 2021 5,435 2 View

Please. Could you help be in implementing Fractal Discrete Cosine Transform algorithm in Matlab?

I do need the Matlab code of Fractal Discrete Cosine Transform (FDCT). Can anyone who has already implement this code, help me with the implementation of this transform?

24 February 2021 5,602 2 View

D2D implementation in Matlab in Vienna LTE software?

Hi Hope you are well. Can you please share your code for D2D implementation in Matlab. I want to implement D2D in Matlab based Vienna simulator and struggling to deploy D2D. Thanks

24 February 2021 9,378 3 View

Multi effect distillation modeling in EES?

Dear colleagues If anyone has a model of multi-effect distillation in EES or MATLAB software, please send it to me.

24 February 2021 7,282 1 View

What program can I use to caclulate dipole moment efficiently and without the need of excessive computation?

Hi, I am a third year chemistry undergrad doing my group project in which I need to predict the HPLC retention time of 20 aromatic molecules. to do this we require the chemical properties of these...

24 February 2021 1,384 3 View

Does anyone ever solved this following Matlab error : "Error using imaqdevice/start (line 95) pointgrey: Failed to stop the camera acquisition." ?

I use Matlab 2014b to capture some images with a PointGrey camera connected with USB3 port. I initialize the camera in a function which basically execute these followed lines : vid =...

22 February 2021 6,386 1 View

How to build fouling and erosion simulation model for Aero Engine compressor and turbine rotor in Simulink / Matlab?

How to build fouling and erosion simulation model for Aero Engine compressor and turbine rotor in Simulink / Matlab?

22 February 2021 3,697 1 View