AAS dried plant samples digestion protocols?

I am looking for alternative sample digestion protocols for dried herbs to be analyzed with AAS for presence of Pb, As and Cd. So far I have attempted acid digestion over hot plate (HNO3 and aqua...

28 December 2020 7,765 5 View

How to draw ROC curve for the correlated parameters?

Dear Fellow Researchers, We want to access the prediction accuracy for various parameters. We were able to draw ROC curves for each of the parameter separately using SPSS. Now, we want to see a...

21 October 2020 4,994 3 View

How can I analyze qPCR data without control sample/calibrator?

I would like to compare expression of my gene of interest (GOI) in non-treated leaves, stems, roots of my plant. In that case I don't have any control sample here and it's not possible to have...

09 August 2020 3,998 3 View

Is it possible that dicot plant could rearrange or excise from his genome transfected DNA fragment?

I have transfected Salvia milthiorrhiza plant using Agrobacterium tumefaciens cells carrying binary plasmid. At the level of Agrobacterium I confirmed presence of my binary vector by obtaining...

26 June 2020 8,763 15 View

Where can I find suppliers of rare dyes?

I have trouble finding certain dyes for my research (mostly acid dyes). I have approached producers of textile dyes from various countries, but the C.I. I am looking for are extremely hard to...

26 March 2020 5,648 6 View

Training and the environment?

Hi, I am looking for current research on training in high polluted air conditions and environment (running, cycling, rowing ...) Effects in plus and in minus (short- and long term). Can anyone...

02 January 2020 3,916 2 View

Any anti-HLA-B38/B39-specific antibodies?

Dear Fellow Researcher, I am working on HLA-B*38 and HLA-B*39 molecules and looking for the antibodies that can recognize these two MHC class I alleles. I do have W6/32 and HC10 antibodies that...

02 December 2019 6,693 1 View

How to evaluate the quality of the studies included in the systematic review?

Dear Fellow Researchers, We are writing a systematic review based on the various types of the studies: clinical trials, clinical case series and the animal studies. What type of study assessment...

17 November 2019 3,131 3 View

Which kit would you recommend for isolation of RNA from dicotyledonous plants (leaves, stems and roots)? Which vendor would you recommend generally?

My plant material is rich in secondary metabolites. With the isolated RNA I would like to examine expression of interested genes in Real Time PCR.

12 November 2019 5,338 8 View

How to efficiently transfer 1.5mm SDS gel on PVDF?

Hello, I have issues with transferring 1.5mm thick SDS-PAGE gels (14% acrylamide, with saccharose) on PVDF membrane. I tried 50 min @ 100V in Towbin buffer with 10% Met-OH, but most of the...

02 July 2019 1,898 4 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...

01 March 2021 2,327 1 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

How to prevent ANFIS Model from negative prediction values in MATLAB?

I have input and output data set for "ANFIS modeling in MATLAB", and I am getting some negative predicted values of output in testing. However, the predicted values of output in training are...

28 February 2021 3,459 3 View

How does an AC-DC power supply Flyback Converter Based USB Charger Model using Matlab/Simulink looks like?

I am required to learn about Flyback converters and I got stuck not knowing to full design of the power supply flyback converter Based USB Charger Model using Simulink, especially the design model...

25 February 2021 5,435 2 View

Please. Could you help be in implementing Fractal Discrete Cosine Transform algorithm in Matlab?

I do need the Matlab code of Fractal Discrete Cosine Transform (FDCT). Can anyone who has already implement this code, help me with the implementation of this transform?

24 February 2021 5,602 2 View

D2D implementation in Matlab in Vienna LTE software?

Hi Hope you are well. Can you please share your code for D2D implementation in Matlab. I want to implement D2D in Matlab based Vienna simulator and struggling to deploy D2D. Thanks

24 February 2021 9,378 3 View

Multi effect distillation modeling in EES?

Dear colleagues If anyone has a model of multi-effect distillation in EES or MATLAB software, please send it to me.

24 February 2021 7,282 1 View

What program can I use to caclulate dipole moment efficiently and without the need of excessive computation?

Hi, I am a third year chemistry undergrad doing my group project in which I need to predict the HPLC retention time of 20 aromatic molecules. to do this we require the chemical properties of these...

24 February 2021 1,384 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View