Hi All, I have seen various papers regarding grafting of Methaacrylate anhydried on Gelatin for photocroslinking. I am curious that Acrylate grafted group has unsaturated Alkene(C=C) end chain, and can we crosslink this Alkene ( c=c) group with primary amine or other functional groups with or (preferably) without photocroslinking method .... Can You suggest me the protocols if Any..
Thanks In Advance
Good afternoon,
Although I work with GelMA, I haven't heard much other chemistry being used on this polymer than radical based polymerization.
However other polymeric methacrylates do have examples of Micheal type crosslinking reactions. For example Hyaluronic acid methacrylate can be crosslinked with DTT:
doi:10.1016/j.ijpharm.2006.05.024
And so can polyethylene glycol diacrylates:
Parlato, M., Reichert, S., Barney, N., & Murphy, W. L. (2014). Poly(ethylene glycol) Hydrogels with Adaptable Mechanical and Degradation Properties for Use in Biomedical Applications. Macromolecular Bioscience, 14(5), 687–698.
Although there is no guarantee this will work with GelMA, there is a fair chance it will, as the crosslinking chemistry is the same.
Good luck!
Karolis
Gelatin acrylate plus PEG divinyl sulfone (or PEG dithiol) may be crosslinked through Micheal-type addition reaction. Gelatin Methacrylate can be less effective than Gelatin acrylate. However, photocrosslinking by U.V is mAdd your answeruch faster than normal chemical crosslinking by chemicals.
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