Fernanda, compound 48/80 is used to induce, not to block, MC degranulation...

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1903762/pdf/amjpathol00215-0040.pdf

Some people are using antibodies :

against mouse FcεRI called mAbMAR-1 (deplete also basophils and FceRI+ DC)

against mouse CD117(Kit) called mAbACK2 (not very usefull)

The best is to use cKITW-Wsh mice lacking mast cells and you reconstitute them with BMMC done in vitro.

I would irradiate, the cKit have problems i.e. no CORIN, the Wsh are your best bet.

If you pre differentiate the mast cells before reconstitution, the mast cells do not return to all tissues. (Galli has a paper on this), but you could reconstitute with whole bone marrow.

If you look at Jax, there may be a flox cre system you cold use.

Patrick,

I think this will be quite difficult since mast cells are positioned in tissues... and I am anyways very skeptic about mouse models with respect to basophils or mast cells because these cells are functionally different from their human counterparts. Then the problem of functional differences of these cells between BL6 and Balb/c... (which one compares best with the human system??).

Would it be better for your purpose to block some mast cell mediator instead? Why do you want to deplete mast cells? I think there are also basophil knockout mice.

Good luck!

If you suspect that the mast cell molecule(s) that is relevant for your system is contained in granules, you may inhibit MC degranulation instead of depleting MC, using sodium cromoglycate (cromolyn). Have a look here

http://cancerres.aacrjournals.org/content/71/18/5987.long

Thanks for all your reply, I'm checking the role of mast cell in certain disease by W/Wv mice.

But there're just so many other unwanted phenotype like anemia. So I want to check if there're

some other ways to deplete. Blocking MC degranulation sounds to be a promising method, I'll

give it a shot

May be its worth looking at http://www.cell.com/immunity/retrieve/pii/S1074761311004614 for a start.

I would suggest you to search for using anti-CD300 to block mast cell degranulation.

Another drug that can be used in vivo without problem to block mast cell degranulation is compount 48/80.

This is a basic article on the subject:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1509129/pdf/bripharmchem00119-0150.pdf

Fernanda, compound 48/80 is used to induce, not to block, MC degranulation...

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1903762/pdf/amjpathol00215-0040.pdf

It is true!! Sorry guys!

But some protocols used C48/80 as a chronic treatment before the assay for research the mast cell participation on process. The intention is finish with the mast cell content.

One option is to use mast cell deficient mice....

Dear Fernanda, I was shocked while saw your recommendations regarding 48/80. By the way those protocols should be avoided. wish you all the best!

Have a look on this paper

http://www.nature.com/labinvest/journal/v92/n10/full/labinvest2012116a.html

It seems that cromolyn effectiveness and selectivity in mice is not that good...

Do you want to deplete mouse or human mast cells ?