Would you spare 10 minutes to fill out a survey on COVID-19?

01 July 2020 8,722 13 View

Do you have time for a quick survey on psychological Factors and COVID-19?

18 June 2020 1,547 7 View

Remove antibody from coimmunoprecipitation mixture?

Recently, I found a commercial kit (or reagent), by which in immunoprecipitation, it removes the precipitating antibody from the mixture and in WB you will not see the 50 and 100 kDa bands....

03 March 2019 3,494 3 View

Cracks in thermally evaporated Gold layer?

Hello everyone, I am using thermal evaporator to deposit 70-100 nm Gold (Au) layer on top of FTO substrate. The gold layer always shows lots of cracks. I have attached a photo here. I ve tried Si...

03 April 2018 2,748 5 View

How can antibodies detect SRY (sex-determining region Y) protein?

Subcellular location of SRY (sex-determining region Y) protein is in the nucleus and cytoplasm of Y-chromosome bearing sperm (http://www.uniprot.org/uniprot/Q03255). So how can antibodies act...

21 February 2017 5,850 1 View

Is it possible to model beams (using beam elements) that have non uniform section, as for instance an increasing cross section?

Hi, If yes, how can I implement it? Thanks

07 August 2014 332 4 View

How can I find the type of oxides present and their percentage based on EDX elemental analysis of a surface?

SEM/EDX Analysis

03 November 2013 5,182 8 View

Can we trust protein molecular weight markers to detect the presence of the right protein in Western blot?

In Western blots sometimes we get a band upper or lower than what expected. Can we trust these bands to verify the presence of a protein?

17 September 2013 2,184 2 View

What do you think about using only secoundary conjugated Ab in indirect flow cytometry when you do not have the proper unconjugated isotype control?

The textbooks say we should always use the same concentration of isotype control as the test monoclonal Ab in flow cytometry assays to detect surface expression of a molecule. In my experiment,...

18 December 2012 4,398 21 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What are anisotropic electrical and optical properties of a semiconductor due to?

In some materials, properties such as band gap, thermal conductivity differ along different crystallographic axes. What are the basics?

01 March 2021 1,923 3 View

Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

Can impedance of antenna be less than 50 ohms?

I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...

27 February 2021 4,218 7 View

Should we reduce the standard unit of length to 0.31951540592 meter ?

The reasoning behind this question is to align the ratios of the proton potential in energy per charge to the speed of light in distance per time. As these two dimensions appear to be related,...

27 February 2021 947 7 View

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

No title

I am trying to find how to know the density needed for a pva electrospinning in a 0.6mm neddle, the electrospinning will be from top to bottom and the problem I really have is the solution because...

25 February 2021 2,615 3 View

Modeling viscosity using model of Chung?

Hello, As part of simulation of gases mixture and water, I need to calculate viscosity of the fluid components and I am using relationships proposed by Chung et al. (1988). The irony is while I...

25 February 2021 8,053 5 View