01 January 1970 0 7K Report

Does anybody know how put IRES and two MCS in a vector to express two genes simultaneously? The second question is what vector is better to put these pieces in it, for example, pcDNA or any other vector?

More Faezeh Jamechenarboo's questions See All
Equation of calculating ammonia nitrogen?

i was determined ammonia nitrogen by phenol hypocholorite method, now i have some data like wieght of sample and absorption level at 630 nm and standard curve and standard equation obtained from...

15 May 2024 2,096 2 View

How can I calculate Gibbs free energy for ligand-protein in Schrödinger?

I have done the docking process using Schrödinger for several ligand-proteins, how to calculate the value of ΔG for each of the ligand-proteins? regards

19 February 2024 5,869 3 View

Can the solution of the Fokker-Planck equation exhibit negative values?

Is it possible for the solution of the Fokker-Planck equation to have negative values? I am referring to the mathematical aspect, irrespective of its physical interpretation. Additionally,...

25 December 2023 560 3 View

I have the problem whit cell uptake?

Hi everyone I synthesized carbon dot with folic acid and citric acid with DMF solvent. After analyzing, it was found that there is no problem with the synthesis. But using a fluorescent...

23 December 2023 4,111 3 View

What is the reason behind the substantial underutilization of oral anticoagulation in patients with atrial fibrillation who are undergoing TAVR?

Oral anticoagulation reduces thromboembolic events in patients with nonvalvular atrial fibrillation (AF); however, underuse of anticoagulation is a major issue in treating patients with...

05 August 2023 3,771 3 View

Calculate the price of beef production??

hi can anyone guide me to calculate the price of beef production like introducing books about it and calculation formulas, please?? I need to get the costs of beef production at the time of breeding.

10 July 2023 7,217 5 View

(new or) innovative technology in ruminant feed??

hi can anyone introduce a new technology or an article about ruminant feed, please? thank you.

05 May 2023 7,040 7 View

Dose anyone have the full text of Official Methods of Analysis of AOAC Internationa ??

Hi I need that for my dissertation, but I can't find a file about it. Can anyone guide me, please??

03 May 2023 9,719 2 View

How can I remove a wrong article from homepage while it is not available in profile anymore?

Hello Few days a go I inserted an article into the ResearchGate, one of author's name has been inserted wrong. I have already deleted the article from my profile but it is still visible in my home...

03 April 2023 667 1 View

Can anyone recommend a book on digestion kinetics in ruminants?

i need to know more about rumen digestion.

08 March 2023 3,605 12 View

Similar questions and discussions
How to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field in Techplot?

Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You

02 August 2024 3,615 1 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Consequences of excess bases in the MCS after digestion?

Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...

17 July 2024 2,787 4 View

I need help with cloning a 200 bp insert and a 4,7 Kb pEGFP-N1 vector. How can I do it?

I've been trying to clone my N-terminal inserts in the comercial pEGFP-N1 vector. Initially, I cloned my N-terminal insert into a pGEMT-easy vector to ensure that the insert digestion was done...

09 July 2024 4,277 1 View

Cloning restrction digestion needed before sequencing?

Hello, recently i am an issue swith cloning where I am using the subclone method to insert my gene length is 1.5kb and my vector(pMAL-c6T) is 5kb I got the colonies which i screened through the...

04 July 2024 7,977 2 View

Why I am unable to get the expected insert at the cloning site?

I am trying to clone a gRNA (23 bases) into the Crispr cas9 pDirect23A vector by using restriction enzyme cloning. I have performed a complete experiment, starting with plasmid isolation,...

02 July 2024 5,448 2 View

What could be the expression profile of alkaline phosphatase from lactic acid bacteria?

I have already cloned ALP gene of L rhamnosus into pET22b+ vector. Cloning is already confirmed by restriction digestion and sequencing. Cloning is achieved in BL21(DE3), Codon+, Rosetta and Lemo...

02 July 2024 5,332 3 View

List Plasmid suitable for Homology cassette in CRISPR-Cas9 protocol for Knock-In in mammalian cell line ?

Hello, I am working on creating a mutant endothelial cell line having Neomycin knock-in present, I have designed my homology arms of 1kb size having neomycin in between them, I would like to...

25 June 2024 1,197 0 View

How can I convert remote sensing (RS) images to vector data automatically?

I am working on a project that involves analyzing remote sensing (RS) images. I need to convert these images into vector data for further analysis. I am looking for automated methods or software...

22 June 2024 6,165 2 View

Asking for a vector, containing Hygromycin B resistance gene?

In my work, Hygromycin B resistance gene is require for screening. I tried to amplify this gene from the vector pIJ941 but failed. I will be thankful, if anyone help me out from this problem by...

19 June 2024 7,822 3 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close