While isolating primary cardiomyocytes from neonatal mice/rat, even after pre-plating the cell suspension the number of fibroblast remaining with the cardiomycoytes is very large. Iam pre-plating the cell suspension in horse serum after enzymatic digestion and leaving it for 2-3 hours in the incubator at 37 degree celcius. How can I reduce these fibroblasts in Cardiomyocyte culture without using inhibitors for fibroblast?
@Bin - we stained with alpha sarcomeric actinin, saw there were more number of nuclei then the actinin stained cells.
@Helene- Ok. Could you tell me what is the approximate fraction of the cardiomyocytes in cell population with this method?
@Noelia- we use horse serum
@Adi I have approximately at least 80% of beating cardiomyocytes in my culture. We use this kit for years and I think method and reagents used, are easily reproducible for cheap homemade techniques. Sorry for answering so late. Good luck !
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