When taking pictures, there seems to be a loss of lipids, and I am unable to determine the cause. Could you help me understand why this is happening?
There are no issues until differentiation, so I am wondering if this could be due to improper fixation during the staining process? Alternatively, is it possible that the lipids are being lost during step 5 of the process?
The protocol I followed is as below:
I don't believe the problem lies with the dye, as I prepared the Oil Red O working solution according to the instructions:
Mix 6 ml of Oil Red O stock solution with 4 ml of ddH2O. Let it sit at room temperature for 20 min, then filter it (0.2 µm).
Could you provide any insights or suggestions on what might be causing the lipid loss during imaging?
Dear Ms Kang
You can try the protocol below.
Preparation of stain: 0.5g oil red O is added to 100 ml of 98% isopropanol. 6ml of stock solution is diluted with 4ml water, and after 30min, it is filtered.
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