Looking for tips and tricks to get iPSC-derived human motor neurons to stick to the plate?

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Refik Kanjhan

Dear Susie,

Have you tried coated plastic coverslips? iPS cells usually adhere easily on plastic cover slips coated with either laminin or poly-l-lysine. Make sure that you give them several days to settle down.

best wishes, Refik

Susie Slepian

Thank you Refik, I have not tried poly-l-lysine. I'll look into that

Sighild Lemarchant

Hi Susie,

I used poly-L-ornithine 2h in the incubator, then you remove it and after that, you coat with matrigel for 1 hour (or more) at room temperature, that you will remove right before plating motoneurons, it works well.

Good luck!