Me again :) what i forgot to say is that i have to put it in before five to twelve midnight on Monday.Help ! Please..

Susie

Hello

may be late, however, you can follow these publications:

Article A simple gas production method using a pressure transducer t...

Article Methods for Detecting Microbial Methane Production and Consu...

and special issue of Animal Feed Science and Technology,2005 on The in vitro Gas Production Technique: Limitations and Opportunities

https://www.sciencedirect.com/journal/animal-feed-science-and-technology/vol/123/part/P1

Susi,

How is this test being physically structured?

Hi, in summary what I am doing is using rumen fluid, methanogens by pure culture, growing in Hungate tubes and then using GC. That's where I am getting confused. What would you separate? Or is there some way to measure the concentration by marker level? If it's VFA how did they get there?

Susie

Dear Susi,

I just read your question.I didn't really understand what is your aim. However, commercially there are different solutions for gas production with rumen inoculum, systems that allow you to collect the gas produced during an in vitro fermentation and therefore analyze it for methane concentration. They might be too expensive, so in alternative I can suggest the Hohenheim gas test. It is a very simple method and not expensive. If you need more information let me know.

Federico

The amount of methane is going to be a function of the pH. Above 6.5 the methane production increases. Below 6.5 it rapidly decreases. In our commercial process we use a methane detector/sensor.