Is there any example of insect which was previously reported as non-vector of virus and then able to transmit the same virus in later investigations ?

I am searching for any example insect species of the plant virus which was previously reported as non-vector the in later investigation it was found to be good vector of same virus, due to Genetic...

03 April 2016 6,585 3 View

Can we report virus on partial RdRP gene sequence?

I have a partial sequence of RdRp gene from Carrot Mottle Virus (CMoV, Umbravirus) and Carrot red leaf virus associated RNA virus (CtRLVaRNA, unassigned). They have 81% and 75% ID with database...

04 May 2014 4,231 4 View

Does the melt curve difference depend upon number of copied that are present in plasmid/cDNA or just on the product sequence difference?

I have used primer set F1R1 for qPCR with syber green, in that I used 1ul Plasmid as positive control of published sequence and my cDNA as target. The Ct value for Plasmids was 18 and for cDNA was...

03 April 2014 9,740 2 View

What is the difference between satellite and associated RNA viruses?

Are satellite RNA viruses and polioviruses associated or different? Do they have a different genomic structure?

10 November 2013 2,869 1 View

What are the alternatives for Standard Curve in Quantitative RT-qPCR of RNA Virus instead of In Vitro Transcript?

I am trying to do the InVitro Transcript, but it's not working for me. I have only 2 months remaining for my research in the USA. Can I try something else like cDNA or total RNA to draw the Std...

01 February 2013 624 5 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

No title

02 March 2021 3,060 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View