Hi everyone, i'm currently looking for ifn alpha and beta rtqPCR primer for golden hamster, does anyone have ever used some?
Best
Hello everyone I am working on amplicons for species delimitation on corals. My supervisor want me to make it through a 4 primers PCR on 4 loci (CR, ITS, ORF and ATPSbeta) I have been trying...
01 March 2024 5,288 3 View
The planned relocation of the seat of Indonesia’s government was announced by President Joko Widodo in 2019 and Indonesia's parliament passed a law enabling that in 2022. Home to more than 11...
29 February 2024 6,869 1 View
Although industrial stakeholders are one of the large producers of PFAS, it is important to note that they are not the only source of PFAS in the influent to a wastewater treatment plant. Consumer...
12 February 2024 5,176 0 View
Tuesday, Oct. 3, the Nobel Prize committee announced the 2023 physics prize, recognizing Pierre Agostini, Ferenc Krausz, and Anne L'Huillier "for experimental methods that generate attosecond...
07 January 2024 8,326 2 View
I am pursuing my PhD and chose the topic of pros/cons and ethical concerns in leveraging Artificial Intelligence tools to increase Equity in Performance Management/Appraisal and did not find much...
18 November 2023 8,460 2 View
i need to know how to give my self schizophrenia TEMPORARILY!!!
15 November 2023 7,226 3 View
The 2030 Agenda for Sustainable Development that the 193 United Nations Member States adopted on September 25, 2015 looks to reconcile "people, planet, and profit", acting through 17 Sustainable...
05 November 2023 8,648 2 View
I am looking for research papers published after 2018 that compare the relevance of Google Scholar (using it directly or via Publish or Perish) to other scientific bibliographic databases (such as...
16 August 2023 9,707 0 View
We want to understand what kind of relevent use can be made of Chat GPT for Researchers. We want to share ours expierience about that.
23 May 2023 2,163 4 View
Hello everyone, for a couple of weeks now i try to realize a PCR amplification of DNA extract from coral of the species Pocillopora.sp using ITS2 primer (internal transcribed spacer region 2) for...
12 April 2023 4,869 5 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...
01 August 2024 4,606 7 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
I've seen articles that primarily focus on alpha and beta activity in the frontal regions, but these studies often compare healthy subjects with those having various pathologies. I haven't seen a...
31 July 2024 7,259 1 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...
30 July 2024 2,867 0 View
Dear Researchers, Is it feasible to distinguish between alpha and alpha prime martensite in the Ti6Al4V alloy under rapid solidification in the as-cast state using XRD, in addition to...
30 July 2024 3,849 0 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View