Hello everyone, I'm trying to find a method for quantifying essential amino acids in milk using gc/ms or HPLC/UV/FID. I found a technical note by the American Dairy Science Association with a single method for all the AA that I'm interested in including TRP.
However, the hydrolysis method in the article uses capillary tubes in vacuum, which i have never used and couldn't find practical information online (Like how do i get an accurate amount of sample inside the tube, what equipment to use etc.)
Has anyone used this method (not necessarily for milk) and is willing to share his/hers protocol with me?
Kindly check https://www.researchgate.net/publication/328981971_Determination_of_Amino_Acid_Composition_of_Cow's_Milk_by_Liquid_Chromatography_Using_Precolumn_Derivatization
Also check https://www.researchgate.net/publication/44622343_Technical_note_Rapid_method_for_determination_of_amino_acids_in_milk
The milk's amino acids were measured using a machine Amino acid analysis
Article Technical note: Rapid method for determination of amino acids in milk
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