Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Do you have papers on hindlimb myology of birds to share?

Hi all! we are looking for papers (or information) on muscles of the hindlimbs of birds (extant and extinct) that include the intrinsic muscles of digits. Any help will be more than welcome! Thanks!

22 February 2021 7,186 3 View

Marker Set for 3D Motion Tracking of Upper Arm during Pointing Task?

Does anyone have recommendations of a marker set for 3D motion tracking of the upper arm during a pointing task? Most studies I have come across have focused on reaching. I plan to use Vicon Nexus...

12 January 2021 3,198 1 View

Are there any way to make the comparison between different analysis of reliability possible?

10 December 2020 7,922 4 View

How do I calculate variance explained by each predictor in a multiple regression via SPSS?

I have 4 independent variables within a multiple regression model. I am trying to figure out the percentage of variance each individual independent variable contributes to the model using SPSS....

28 November 2020 583 3 View

Does anyone has suggestions to extract carbohydrates from E. coli?

Dear colleagues, We are interested in extracting carbohydrates fro E. coli and the protocol we've seen so far include too many hours in a sonicator. Does anyone has suggestions or experinece in...

26 November 2020 5,907 3 View

Is the statistical analysis performed correct?

Dear colleagues, I have some doubts about the correctness of the following analysis. A class of 79 students underwent three different teaching methods that chronologically presented themselves...

02 November 2020 4,030 4 View

Is there a validated english questionnaire to assess Achievement Motivation in Sport (Hope for Success and Fear of Failure)?

I'm looking for an instrument in english that does not measure goal orientations.

04 October 2020 3,255 5 View

How to calculate a-priori sample size for a repeated measures 2x2 ANCOVA?

I am planning an experiment 2x2 within-subjects factors and additionally I would like to consider a covariate, so that a 2x2 repeated measures ANCOVA is the intended analysis. Does anyone know how...

16 August 2020 985 4 View

How should I present microRNA expression data from a single sample (leaves)?

Hello! I have a small RNA-seq analysis from leaves, and I will validate the known and the novel miRNAs by qPCR. My question is how I should present the qPCR results from a single sample (leaves)...

30 July 2020 9,696 3 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

Why cannot world and community leaders be gentle? Where did ethics of care go?

The whole world is in a mess and paused. We all are tired of seeing hypermasculinized and destructive leaderships all around us. This world has seen enough of the aggressive patriarchal social...

23 February 2021 292 7 View

What is the most prevalent drug related problem in the developed countries?

Detecting the drug related problems is an important step in the pharmaceutical care plan

21 February 2021 5,421 3 View

Can I simply dilute 25% NH3 solution to 10% solution by pouring water in tank and circulating with pump?

Is there any heat of dilution which I need to take care by putting some circulation cooler? Will there be any significant ammonia emission in the process?

09 February 2021 5,943 2 View

Why marketers need a forensic understanding of consumers and a creative, agile mindset?

02 February 2021 6,579 10 View

When is the right time to stop training a classification algorithm?

In this example giving 400 samples looks like there is an accuracy of 91.5% while training beyond that point, accuracy is getting decreased. Are 400 samples enough to keep for this example? If...

27 January 2021 5,657 3 View

What contributions could be done in the medical emergency field based on voice recognition and NLP?

Hello, My research work focuses on the use of NLP and voice recognition for the medical emergency assistance. So I would appreciate it if you suggest me some contributions that could be done in...

27 January 2021 2,899 3 View

What are the IT and Artificial intelligence contributions to assisting medical emergency cases?

Hello, I am seeking recommendations from the Research Gate community regarding studies about the IT and Artificial intelligence solutions to assisting medical emergency cases. What are the...

23 January 2021 2,667 21 View

I am looking to develop a basic oral microbiome assay. Can anyone advise on culture vessel, conditions and organisms that require minimal maintenance?

I am looking to investigate oral care products with an easy-to-prepare culture of orally derrived microorganisms.

21 January 2021 3,448 1 View

How has Artificial Intelligence impacted Healthcare?

The evolution of Artificial Intelligence is influencing healthcare. With the production of applications such as AI-based biomedical applications, and medical AI systems, etc. Without a doubt, AI...

20 January 2021 3,971 11 View