I'm looking for a protocol for TCR cloning into a retroviral vector with peptide-stimulated T cells as starting point.

I was told by a colleague to first clone the TCR alpha and beta chain using multiple PCR rounds. However, I noticed that there are many different and new methods published.

Does anyone have any preference or recommendation which method/protocol to use? Also which retroviral vector?

Thanks, any feedback is highly appreciated.

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