Is my initial AP FRET set up suitable?

Hi all! I want to use acceptor photobleaching FRET to look at a potential interaction between two proteins of interest. We have a Leica SP8 in my lab that I have used to try and get an idea if...

09 March 2020 2,349 2 View

Is serum diluted for clinical human Anti-idiotypic ELISAs?

I am working on an anti-idiotypic ELISA for human clinical trials and would like to know if human serum is diluted in these assays or used neat. My ab of interest is an IgG4.

01 August 2018 4,708 4 View

Housekeeping proteins/loading controls for a Western Blot of E. coli cell lysates?

18 July 2017 7,679 5 View

What's your favourite mCherry antibody for immunoprecipitation (IP)?

15 January 2017 5,879 3 View

Has length of survival of lab strains of Drosophila changed over the last 50 years?

I am looking for as many different survival curves for D. melanogaster as I can collect. Have many from the literature, but if anyone has survival curve data that they are willing to share that is...

24 August 2016 7,472 1 View

What is the best way to measure glyphosate?

I am looking for either a lab to do the testing or a relatively simple-to-use kit to do it myself. Can it be done on whole body tissues? What quantity of tissue is required? How expensive is this...

03 February 2016 1,367 3 View

Can anyone offer advice on sarkosyl insoluble tau preparations?

25 January 2016 6,634 1 View

Does anyone know if Santa-Cruz Biotechnologies CRISPR/Cas9 plasmids can be transfected with reagents other than the UltraCruz Transfection Reagent?

03 November 2015 5,360 2 View

Does anyone know if CypHer5 mono ester dye is stable after fixation?

I'm hoping to use CypHer5 mono ester dye to label bacteria, then infect cells with the labeled bacteria. I need to do a fixation step before microscopy, and I was wondering if the dye will be...

18 June 2015 2,581 2 View

Can anyone help me solve EMSA competition, shift and supershift problems?

I am trying to do EMSAs and I am using a consensus and mutant oligo specific for my protein which I ordered from Santa Cruz and then a custom biotin labeled oligo (I have annealed sense and...

14 December 2013 2,352 8 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

What are the harmful effects of these extreme rainfall on the health of newborns? What are the reasons for that?

Extreme rainfall associated with climate change is causing harm to babies in some of the most forgotten places.

01 March 2021 5,647 4 View

Is Journal of Agricultural Research a predator?

it is published by Juniper

28 February 2021 354 4 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Could the "incoming radiation (net radiation)" values be negative?

Could the "incoming solar radiation" values be negative? What is the difference between "global solar radiation" and "incoming solar radiation"? Which one might be measured negative values?

21 February 2021 7,726 9 View

Is the box plot of climatic indices better illustrate from daily data or annual data?

Hello, I hope you have a good time. I work on a research project about temperature indices. Due to the high number of indices, I only work on tables and maps on an annual time scale. In other...

19 February 2021 9,742 2 View