I am looking for someone who can help us with H&E staining of ~150 slides (mouse, fish, quail, and toad colons) that have been professionally prepared and embedded in paraffin mounts. I have another ~150 identical slides that I would like to get stained with Alcian Blue also, so we can visualize the mucin layers too if that is possible. I can pay for the costs of staining, but would prefer to offer someone a collaborative role in the manuscript that will be generated from this study. Any help would be appreciated.
Dear Frine, we can do that. I'll be back in office on January 6th. Best regards Torsten
Why not to combine the three staining on the same slides? Hematoxylin, Fushine acid and alcian blue work fine together on the same slide.
Dear Dr, Marshall, my lab is available to collaborate in this research. I have also experience of histochemistry with Lectins for the characterization of mucins and IHC with MUC1, 2, Gal3, etc.
You need only of the "technical" part of the work or even the reading of slides and prepared tables Tab with semiquantitative scores of the different parameters??
Let me know if I can help.
Hello to you soon. Giacomo.
Hello Mashal I am a lecturer in histology in Iran.I and my friend(holder phd in histology fromParis university) could help you,but I should talk to my friend.
my e.mail is [email protected] please contact me for details
Hi Mr. McCum
My name is Cynthia James and I am a registered Histologist. I have 31 years of histology experience(tissue processing, embedding, sectioning, and staining.
Please contact me at [email protected] or 3129199887. I can help you with your project.
We have found a person who is going to do the H&E staining and help with the interpretation. We may be interested in staining and studying the mucins on a second copy of slides in a couple of months, and I'll let you know as that aspect of the study progresses so you can see if you are available for collaboration.
Hi, perfect!
When you are ready with the sections and H&E stain, we feel and we agree to make a determination on mucins. Let me know when you have material available.
See you soon. Have a good work!
Giacomo.
Dear Frine
I is since 1983 working in the field of histology is normal and diseased animals and humans (all organs), particularly the nervous system and gut, you can see my profile on ResearchGATE to see if it reflects what you're looking for .
The H & E staining with the added step of Alcian blue is a generic staining if you have to identify some specific mucopolysaccharides you must use the Alcian blue in critical electrolytical concetration , ie, at different pH for to identify specific mucopolysaccharides. Other staining that we do make is galgano staining the is made of five dye and mucin is colored blue.
Best regards
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